Use of the alkaline in vivo Comet assay for mechanistic genotoxicity investigations
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چکیده
منابع مشابه
Evaluation of in vivo genotoxicity of cypermethrin in Drosophila melanogaster using the alkaline Comet assay.
The single cell gel electrophoresis (SCGE) assay, also known as the Comet assay, is one of the most promising genotoxicity tests developed in recent years to measure and analyse DNA damage in single cells. The present study was undertaken to assess the in vivo genotoxicity of the synthetic pyrethroid cypermethrin in brain ganglia and anterior mid gut of Drosophila melanogaster. Freshly emerged ...
متن کاملRecommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop.
The in vivo alkaline single cell gel electrophoresis assay, hereafter the Comet assay, can be used to investigate the genotoxicity of industrial chemicals, biocides, agrochemicals and pharmaceuticals. The major advantages of this assay include the relative ease of application to any tissue of interest, the detection of multiple classes of DNA damage and the generation of data at the level of th...
متن کاملValidation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay.
The single cell gel electrophoresis or Comet assay is one of the most popular techniques for genotoxicity assessment. The present study was undertaken to validate our previously modified version of the Comet assay for genotoxicity assessment in Drosophila melanogaster (Oregon R(+)) with four well-known mutagenic and carcinogenic alkylating agents, i.e. ethyl methanesulfonate (EMS), methyl metha...
متن کاملGenotoxicity of cadmium in marine diatom Chaetoceros tenuissimus using the alkaline Comet assay.
Genotoxic effects of cadmium on phytoplankton Chaetoceros tenuissimus have been evaluated using DNA damage by Comet assay. Cadmium concentrations ranging from 2.4 to 10 mg/l were used to evaluate the effects. Results showed that as the concentration of Cd increased growth of the diatom decreased. Alkaline single-cell gel electrophoresis (Comet assay) method, which is highly sensitive in detecti...
متن کاملThe use of the comet assay for the evaluation of the genotoxicity of nanomaterials
The accelerating production and use of engineered nanomaterials (NMs) raises questions about the safety of this new technology. To avoid the possible hazards associated with NMs requires proper regulation. How should toxicity testing be addressed and can standard tests for assessment of safety of chemicals be applied to NMs? What are the major limitations of NM safety testing and is the current...
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ژورنال
عنوان ژورنال: Mutagenesis
سال: 2004
ISSN: 1464-3804
DOI: 10.1093/mutage/geg038